Research Professor
Frank Reidy Research Center for Bioelectrics

Andrei Pakhomov

757-683-8003

SUITE 300 4211 MONARCH WAY
NORFOLK, 23508

Ph.D. in Biophysics/Radiation Biology, Medical Radiology Research Center, (1989)

M.Sc. in Biology/Animal and Human Physiology, Moscow State University, (1982)

Contracts, Grants and Sponsored Research

"Nanoelectropulse-induced electromechanical signaling and control of biological systems" $7,500,000. Federal. October 01, 2015 - September 30, 2020
"Low Energy Defibrillation with Nanosecond Pulsed Electric Field" $1,509,283. Federal. August 12, 2015 - May 31, 2019
Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $611,260. Federal. September 30, 2015 - September 30, 2018
Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $142,937. Federal. September 30, 2015 - September 30, 2018
Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $136,579. Federal. September 30, 2015 - September 30, 2018
Pakhomov, A., Pakhomova, O. and Semenov, I. "Nanoelectropulse-Induced Electromechanical Signaling And Control of Biological Systems" $132,941. Federal. September 30, 2015 - September 30, 2018
Pakhomov, A., Pakhomova, O., Semenov, I., Hill, E. L., Lobova, T. and Morris, J. C. "Low Energy Defibrillation With Nanosecond Pulsed Electric Field" $151,075. Federal. June 1, 2016 - May 31, 2017
Xiao, S. "Picosecond pulse technology for non-invasive electrostimulation" $393,393. Federal. March 1, 2014 - February 28, 2017
Pakhomov, A., Pakhomova, O. and Xiao, S. "Mechanisms and Implications of Nanoelectroporation in living cells" $1,200,000. Federal. July 10, 2010 - June 30, 2015
Zemlin, C. "Defibrillation of Cardiac Tissue with Nanosecond Pulsed Electric Fields" $50,000. Old Dominion University. 2013 - 2014
Ibey, B. and Pakhomov, A. "Investigation of field-dependent bioeffects of high amplitude electromagnetic pulses" $1,730,000. Federal. 2011 - 2013
Pakhomov, A. and Ibey, B. "Mechanism of interaction between pulsed electric field and phospholipid bilayer membrane" $623,000. Federal. 2009 - 2013
Pakhomova, O. and Xiao, S. "Cell Death Induction by High-Voltage, Nanosecond-Duration Electric Pulses" $1,078,970. Federal. 2008 - 2013
Pakhomov, A., Beebe, S. J., Swanson, R. J., Xiao, S. and Schoenbach, K. H. "A Comprehensive Study of Neuromuscular Effects of Nanosecond Electrical Pulses" $1,967,101. Federal. 2007 - 2010

Research Interests

Electrophysiology
Electroporation
Membrane biology
Cell biology

Articles

Semenov, I., Grigoryev, S., Neuber, J. U., Zemlin, C., Pakhomova, O., Casciola, M. and Pakhomov, A. (2018). Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks. Scientific Reports 8 , pp. 12.
Pakhomov, A., Grigoryev, S., Semenov, I., Casciola, M., Jiang, C. and Xiao, S. (2018). The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency. Bioelectrochemistry 122 , pp. 123-133.
Gianulis, E. C., Casciola, M., Xiao, S., Pakhomova, O. and Pakhomov, A. (2018). Electropermeabilization by uni- or bipolar nanosecond electric pulses: The impact of extracellular conductivity.. Bioelectrochemistry (Amsterdam, Netherlands) 119 , pp. 10-19.
Muratori, C., Pakhomov, A., Gianulis, E., Meads, J., Casciola, M., Mollica, P. A. and Pakhomova, O. (2017). Activation of the phospholipid scramblase tmem16f by nanosecond pulsed electric field (nspef) facilitates its diverse cytophysiological effects. Journal of Biological Chemistry.
Varghese, F., Neuber, J. U., Xie, F., Philpott, J. M., Pakhomov, A. and Zemlin, C. (2017). Low-energy defibrillation with nanosecond electric shocks.. Cardiovascular research 113 (14) , pp. 1789-1797.
Muratori, C., Pakhomov, A., Gianulis, E., Meads, J., Casciola, M., Mollica, P. A. and Pakhomova, O. (2017). Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.. The Journal of biological chemistry 292 (47) , pp. 19381-19391.
Jensen, S. D., Khorokhorina, V. A., Muratori, C., Pakhomov, A. and Pakhomova, O. (2017). Delayed hypersensitivity to nanosecond pulsed electric field in electroporated cells.. Scientific reports 7 (1) , pp. 10992.
Casciola, M., Xiao, S. and Pakhomov, A. (2017). Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.. Scientific reports 7 (1) , pp. 10453.
Xiao, S., Semenov, I., Petrella, R., Pakhomov, A. and Schoenbach, K. H. (2017). A subnanosecond electric pulse exposure system for biological cells.. Medical & biological engineering & computing 55 (7) , pp. 1063-1072.
Merla, C., Pakhomov, A., Semenov, I. and Vernier, T. (2017). Frequency spectrum of induced transmembrane potential and permeabilization efficacy of bipolar electric pulses.. Biochimica et biophysica acta 1859 (7) , pp. 1282-1290.
Pakhomov, A., Semenov, I., Casciola, M. and Xiao, S. (2017). Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.. Biochimica et biophysica acta 1859 (7) , pp. 1273-1281.
Gianulis, E. C., Labib, C., Saulis, G., Novickij, V., Pakhomova, O. and Pakhomov, A. (2017). Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.. Cellular and molecular life sciences : CMLS 74 (9) , pp. 1741-1754.
Muratori, C., Pakhomov, A. and Pakhomova, O. (2017). Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.. The Journal of membrane biology 250 (2) , pp. 217-224.
Muratori, C., Pakhomov, A., Heller, L., Casciola, M., Gianulis, E., Grigoryev, S., Xiao, S. and Pakhomova, O. (2017). Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric Fields In Vivo.. Technology in cancer research & treatment , pp. 1533034617712397.
Muratori, C., Pakhomov, A., Gianulis, E. C., Jensen, S. D. and Pakhomova, O. (2016). The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.. Scientific reports 6 , pp. 36835.
Dermol, J., Pakhomova, O., Pakhomov, A. and Miklavčič, D. (2016). Cell Electrosensitization Exists Only in Certain Electroporation Buffers.. PloS one 11 (7) , pp. e0159434.
Semenov, I., Xiao, S. and Pakhomov, A. (2016). Electroporation by subnanosecond pulses.. Biochemistry and biophysics reports 6 , pp. 253-259.
Muratori, C., Pakhomov, A., Xiao, S. and Pakhomova, O. (2016). Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.. Scientific reports 6 , pp. 23225.
Xie, F., Varghese, F., Pakhomov, A., Semenov, I., Xiao, S., Philpott, J. and Zemlin, C. (2015). Ablation of Myocardial Tissue With Nanosecond Pulsed Electric Fields.. PloS one 10 (12) , pp. e0144833.
Semenov, I., Xiao, S., Kang, D., Schoenbach, K. H. and Pakhomov, A. (2015). Cell stimulation and calcium mobilization by picosecond electric pulses.. Bioelectrochemistry (Amsterdam, Netherlands) 105 , pp. 65-71.
Semenov, I., Zemlin, C., Pakhomova, O., Xiao, S. and Pakhomov, A. (2015). Diffuse, non-polar electropermeabilization and reduced propidium uptake distinguish the effect of nanosecond electric pulses.. Biochimica et biophysica acta 1848 (10 Pt A) , pp. 2118-25.
Vernier, T., Levine, Z. A., Ho, M. C., Xiao, S., Semenov, I. and Pakhomov, A. (2015). Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes.. The Journal of membrane biology 248 (5) , pp. 837-47.
Gianulis, E. C., Lee, J., Jiang, C., Xiao, S., Ibey, B. L. and Pakhomov, A. (2015). Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal.. Scientific reports 5 , pp. 13818.
Schoenbach, K. H., Pakhomov, A., Semenov, I., Xiao, S., Pakhomova, O. and Ibey, B. L. (2015). Ion transport into cells exposed to monopolar and bipolar nanosecond pulses.. Bioelectrochemistry (Amsterdam, Netherlands) 103 , pp. 44-51.
Pakhomov, A., Gianulis, E., Vernier, T., Semenov, I., Xiao, S. and Pakhomova, O. (2015). Multiple nanosecond electric pulses increase the number but not the size of long-lived nanopores in the cell membrane.. Biochimica et biophysica acta 1848 (4) , pp. 958-66.
Gianulis, E. C. and Pakhomov, A. (2015). Gadolinium modifies the cell membrane to inhibit permeabilization by nanosecond electric pulses.. Archives of biochemistry and biophysics 570 , pp. 1-7.
Pakhomov, A., Xiao, S., Pakhomova, O., Semenov, I., Kuipers, M. A. and Ibey, B. L. (2014). Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling.. Bioelectrochemistry (Amsterdam, Netherlands) 100 , pp. 88-95.
Pakhomov, A., Semenov, I., Xiao, S., Pakhomova, O., Gregory, B., Schoenbach, K. H., Ullery, J. C., Beier, H. T., Rajulapati, S. R. and Ibey, B. L. (2014). Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity.. Cellular and molecular life sciences : CMLS 71 (22) , pp. 4431-41.
Pakhomova, O., Gregory, B., Semenov, I. and Pakhomov, A. (2014). Calcium-mediated pore expansion and cell death following nanoelectroporation.. Biochimica et biophysica acta 1838 (10) , pp. 2547-54.
Rassokhin, M. and Pakhomov, A. (2014). Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF).. Cell biochemistry and biophysics 69 (3) , pp. 555-66.
Ibey, B. L., Ullery, J. C., Pakhomova, O., Roth, C. C., Semenov, I., Beier, H. T., Tarango, M., Xiao, S., Schoenbach, K. H. and Pakhomov, A. (2014). Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses.. Biochemical and biophysical research communications 443 (2) , pp. 568-73.
Pakhomova, O., Gregory, B. W.. and Pakhomov, A. (2013). Facilitation of electroporative drug uptake and cell killing by electrosensitization. J Cell Mol Med 17 (1) , pp. 154-9.
Semenov, I., Xiao, S. and Pakhomov, A. (2013). Primary pathways of intracellular Ca(2+) mobilization by nanosecond pulsed electric field. Biochim Biophys Acta 3 , pp. 981-9.
Semenov, I., Xiao, S., Pakhomova, O. and Pakhomov, A. (2013). Recruitment of the intracellular Ca by ultrashort electric stimuli: The impact of pulse duration. Cell Calcium 15 (13) , pp. 00083-3.
Pakhomova , O., Gregory, B., Semenov, I. and Pakhomov, A. (2013). Two modes of cell death caused by exposure to nanosecond pulsed electric field. PLOS One 8 (7) , pp. e70278 .
Nesin, V., Bowman, A. M.., Xiao, S. and Pakhomov, A. (2012). Cell permeabilization and inhibition of voltage-gated Ca(2+) and Na(+) channel currents by nanosecond pulsed electric field. Bioelectromagnetics 33 (5) , pp. 394-404.
Rassokhin, M. and Pakhomov, A. (2012). Electric field exposure triggers and guides formation of pseudopod-like blebs in U937 monocytes. J Membr Biol 245 (9) , pp. 521-9.
Nesin, V. and Pakhomov, A. (2012). Inhibition of voltage-gated Na(+) current by nanosecond pulsed electric field (nsPEF) is not mediated by Na(+) influx or Ca(2+) signaling. Bioelectromagnetics 33 (6) , pp. 443-51.
Pakhomova, O., Khorokhorina, V. A.., Bowman, A. M.., Rodaite-Riseviciene, R., Saulis, G., Xiao, S. and Pakhomov, A. (2012). Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media. Arch Biochem Biophys 527 (1) , pp. 55-64.
Nesin, V. and Pakhomov, A. (2012). Inhibition of voltage-gated Na+ current by nanosecond pulsed electric field (nsPEF) is not mediated by Na+ influx or Ca2+ signaling.. Bioelectromagnetics 33 (6) , pp. 443-51.
Ibey, B. L.., Roth, C. C.., Pakhomov, A., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2011). Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines. PLoS One 6 (1) , pp. 0015642.
Pakhomova, O., Gregory, B. W.., Khorokhorina, V. A.., Bowman, A. M.., Xiao, S. and Pakhomov, A. (2011). Electroporation-induced electrosensitization. PLoS One 6 (2) , pp. 0017100.
Nesin, O. M.., Pakhomova, O., Xiao, S. and Pakhomov, A. (2011). Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. Biochim Biophys Acta 3 , pp. 792-801.
Bowman, A. M.., Nesin, O. M.., Pakhomova, O. and Pakhomov, A. (2010). Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptake. J Membr Biol 236 (1) , pp. 15-26.
Andre, F. M.., Rassokhin, M., Bowman, A. M.. and Pakhomov, A. (2010). Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death. Bioelectrochemistry 79 (1) , pp. 95-100.
Ibey, B. L.., Mixon, D. G.., Payne, J. A.., Bowman, A., Sickendick, K., Wilmink, G. J.., Roach, W. P.. and Pakhomov, A. (2010). Plasma membrane permeabilization by trains of ultrashort electric pulses. Bioelectrochemistry 79 (1) , pp. 114-21.
Ibey, B. L.., Pakhomov, A., Gregory, B. W.., Khorokhorina, V. A.., Roth, C. C.., Rassokhin, M., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2010). Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells. Biochim Biophys Acta 1800 (11) , pp. 1210-9.
Nuccitelli, R., Chen, X., Pakhomov, A., Baldwin, W. H.., Sheikh, S., Pomicter, J. L.., Ren, W., Osgood, C. J., Swanson, R. J., Kolb, J. F., Beebe, S. J. and Schoenbach, K. H. (2009). A new pulsed electric field therapy for melanoma disrupts the tumor’s blood supply and causes complete remission without recurrence. Int J Cancer 125 (2) , pp. 438-45.
Pakhomov, A., Bowman, A. M.., Ibey, B. L.., Andre, F. M.., Pakhomova, O. and Schoenbach, K. H. (2009). Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochem Biophys Res Commun 385 (2) , pp. 181-6.
Ibey, B. L., Xiao, S., Schoenbach, K. H., Murphy, M. R. and Pakhomov, A. (2009). Plasma Membrane Permeabilization by 60 and 600 ns Electric Pulses is Determined by the Absorbed Dose. Bioelectromagnetics 30 (2) , pp. 90-92.
Joshi, R., Mishra, A., Song, J., Pakhomov, A. and Schoenbach, K. H. (2008). Simulation studies of ultrashort, high-intensity electric pulse induced action potential block in whole-animal nerves. IEEE Trans Biomed Eng 55 (4) , pp. 1391-8.
Osgood, C. J., Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Nuccitelli, R., Pakhomov, A., Stacey, M. W., Swanson, R. J., White, J., Xiao, S. and Zhang, J. (2007). Bioelectric effects of intense nanosecond pulses. IEEE Transact Dielectrics Electrical Insul 14 , pp. 1088-1109.
Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Osgood, C. J., Nuccitelli, R., Pakhomov, A., Swanson, R. J., Stacey, M., White, J. A., Xiao, S., Zhang, J., Beebe, S. J., Blackmore, P. F. and Buescher, E. S. (2007). Bioelectric Effects of Nanosecond Pulses. IEEE Trans. Diel. Electr. Insul. 14 (5) , pp. 1088-1109.
Pakhomov, A., Kolb, J. F., White, J. A., Joshi, R., Xiao, S. and Schoenbach, K. H. (2007). Long-Lasting Plasma Membrane Permeabilization in Mammalian Cells by Nanosecond Pulsed Electric Field (nsPEF). Bioelectromagnetics 28 , pp. 655.
Pakhomov, A., Shevin, R., White, J. A.., Kolb, J. F., Pakhomova, O., Joshi, R. and Schoenbach, K. H. (2007). Membrane permeabilization and cell damage by ultrashort electric field shocks. Arch Biochem Biophys 465 (1) , pp. 109-18.
Pakhomov, A., Semenov, I., Brenner, R. and Toney, G. M.. (2006). Hydraulically coupled microejection technique for precise local solution delivery in tissues. J Neurosci Methods 155 (2) , pp. 231-40.
Walker, 3rd, K., Pakhomova, O., Kolb, J. F., Schoenbach, K. H., Stuck, B. E.., Murphy, M. R.. and Pakhomov, A. (2006). Oxygen enhances lethal effect of high-intensity, ultrashort electrical pulses. Bioelectromagnetics 27 (3) , pp. 221-5.
Pakhomov, A., Doyle, J., Stuck, B. E.. and Murphy, M. R.. (2003). Effects of high power microwave pulses on synaptic transmission and long term potentiation in hippocampus. Bioelectromagnetics 24 (3) , pp. 174-81.
Pakhomov, A., Gajsek, P., Allen, L., Stuck, B. E.. and Murphy, M. R.. (2002). Comparison of dose dependences for bioeffects of continuous-wave and high-peak power microwave emissions using gel-suspended cell cultures. Bioelectromagnetics 23 (2) , pp. 158-67.
Gajsek, P., Pakhomov, A. and Klauenberg, B. J.. (2002). Electromagnetic field standards in Central and Eastern European countries: current state and stipulations for international harmonization. Health Phys 82 (4) , pp. 473-83.
Pakhomov, A., Mathur, S. P.., Doyle, J., Stuck, B. E.., Kiel, J. L.. and Murphy, M. R.. (2000). Comparative effects of extremely high power microwave pulses and a brief CW irradiation on pacemaker function in isolated frog heart slices. Bioelectromagnetics 21 (4) , pp. 245-54.
Pakhomov, A., Akyel, Y., Pakhomova, O., Stuck, B. E.. and Murphy, M. R.. (1998). Current state and implications of research on biological effects of millimeter waves: a review of the literature. Bioelectromagnetics 19 (7) , pp. 393-413.
Pakhomov, A., Prol, H. K.., Mathur, S. P.., Akyel, Y. and Campbell, C. (1997). Search for frequency-specific effects of millimeter-wave radiation on isolated nerve function. Bioelectromagnetics 18 (4) , pp. 324-34.
Pakhomov, A., Dubovick, B. V.., Degtyariov, I. G.. and Pronkevich, A. N.. (1995). Microwave influence on the isolated heart function: I. Effect of modulation. Bioelectromagnetics 16 (4) , pp. 241-9.
Pakhomov, A., Dubovick, B. V.., Degtyariov, I. G.. and Pronkevich, A. N.. (1995). Microwave influence on the isolated heart function: II. Combined effect of radiation and some drugs. Bioelectromagnetics 16 (4) , pp. 250-4.

Book Chapters

Pakhomov, A. and Pakhomova, O. (2010). Nanopores: A distinct transmembrane passageway in electroporated cells Advanced Electroporation Techniques in Biology in Medicine (pp. 178–194) Boca Raton: CRC Press.

Organizational Chart